Coding
Part:BBa_K4716986:Design
Designed by: LIAO WENYU Group: iGEM23_UM-Macau (2023-10-10)
PEP-6his tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1747
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 993
Illegal XhoI site found at 1756 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1114
Design Notes
We choose to use the PEP from Aspergillus oryzae RIB40 and do our research together with Fvp-p and compare the effect of both of them to digest the 33-mer toxic peptide of gluten.
Source
This sequence we use is found from the gene bank of NCBI, the source: https://www.ncbi.nlm.nih.gov/nuccore/XM_001825944.2?report=genbank